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FIGURE 2. High nitrate induces OsNPF7.2 expression in roots. (A,B) qPCR analysis of short-term induction of OsNPF7.2 in rice roots under 10 mM (A) and 0.5 mM (B) nitrate solution, respectively. ZH11 seedlings were grown in IRRI solution for 2 weeks, and then transferred for 3-day nitrate starvation, finally shifted in IRRI solution with 10 mM or 0.5 mM KNO3 or KCl instead of original N sources. In the solution, equal molar of KCl instead of KNO3 was used as control. (C) Long-term induction of OsNPF7.2 in rice roots. ZH11 seedlings were grown on 1/2 MS medium with various concentrations of KNO3 for 2 weeks. Ammonium was used to maintain total N concentrations equal in these medium. Data in (A–C) represent mean ± SD from one experiment of 10 seedlings, two independent experiments showed same result. Image published in: Hu R et al. (2016) Copyright © 2016 Hu, Qiu, Chen, Miller, Fan, Pan and Zhang. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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