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FIGURE 3. OsNPF7.2 is mainly localized on tonoplast. (A,B) Rice protoplasts expressing 35S: EGFP as control. (C–F) Rice protoplasts expressing 35S: OsNPF7.2: EGFP and a tonoplast localized marker vac-rk. (G–J) Rice protoplasts expressing 35S: EGFP: OsNPF7.2 and a tonoplast localized marker vac-rk. BF, bright field; GFP, green fluorescent protein. Merged shows the signal of GFP merged with corresponding mCherry. Arrowhead indicates the small vacuoles can be merged with vac-rk. Arrows indicate the small vacuoles cannot be merged with vac-rk. Bar = 5 μm.

Image published in: Hu R et al. (2016)

Copyright © 2016 Hu, Qiu, Chen, Miller, Fan, Pan and Zhang. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license

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