Xenbase Image ID: 155329

Figure 3. ZIP8 knockdown by shRNA causes decreased ZIP8 protein expression and Se and Zn uptakeA. Western blot of human ZIP8 in TAs cells stably expressing shRNA against ZIP8 mRNA (shRNA-1 and shRNA-2) vs scrambled shRNA (sc-shRNA) control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase, lane-loading control. B. Se content and C. Zn content in TAs cells, ZIP8-knockdown vs control, after exposure to HSeO3− + Zn2+ (both 200 μM) for 30 min. TAs_ZIP8-shRNA-1 and TAs_ZIP8-shRNA-2 represent two different stably-expressing cell lines carrying two different shRNAs. For all experiments, HCO3− (25 mM) was present in the culture medium. Intracellular Se and Zn content was determined by ICP-MS. Brackets denote S.E.M. *P <0.05, **P <0.01. Image published in: McDermott JR et al. (2016) Copyright: © 2016 McDermott et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page