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Figure 5. Se content in mouse whole blood or washed red blood cells (RBCs)A. relative Se content in WT whole blood, following HSeO3− treatment (20 μM; 15 min at 37°C), with, versus without, Zn2+ (75 μM); where indicated, whole blood was pretreated for 5 min with EDTA (4 mM) or TPEN (100 μM). Value for selenite-treated whole blood without added Zn2+ was chosen as “1.0” and all other values are relative to that. B. Intracellular Se content in isolated WT RBCs resuspended in PBS containing HCO3− (10 mM; 15 min at 37°C), as a function of Zn2+ concentration following 5-min pretreatment with HSeO3− (20 μM, 37°C). C. Intracellular Se content in isolated RBCs from the three mouse genotypes resuspended in PBS with HCO3− (25 mM) + 75 μM Zn2+ (closed red bars) versus 25 mM HCO3− alone (open bars). Cells were incubated with HSeO3− (20 μM) for 5 min at 37°C. **P <0.01, comparing BTZIP8-3 with, vs without, added Zn2+. D. comparison of rate of Se uptake in RBCs (also resuspended in PBS), as a function of incubation time (37°C); RBCs were isolated from BTZIP8-3, WT and Slc39a8(+/neo) mice; RBCs were treated with HSeO3− (20 μM). **P <0.01, comparing BTZIP8-3 with both WT and Slc39a8(+/neo). Brackets denote S.E.M. Image published in: McDermott JR et al. (2016) Copyright: © 2016 McDermott et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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