XB-IMG-155551
Xenbase Image ID: 155551
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Figure 4. NPF2.5 is localized to the plasma membrane in Arabidopsis. (A) Green fluorescence detected in root cells of 2-week old GFP::NPF2.5 plants. (B) Transmitted light image of root cells of 2-week old GFP::NPF2.5 plants. (C–E) Confocal image of plasmolysis performed on root cells of 2-week GFP::NPF2.5 plants. (F) Yellow florescence of YFP::NPF2.5 fusion protein observed in an Arabidopsis mesophyll protoplast. (G) Cyan fluorescence of plasma membrane marker eCFP::ROP11 observed in the protoplast. (H) Transmitted light image of the protoplast. White arrow points to the separation of the tonoplast from the plasma membrane. (I) Merged image showing co-localisation of yellow fluorescence of YFP::NPF2.5 fusion protein and cyan fluorescence of plasma membrane marker. Scale bars = 50 μm in (A,B); Scale bars = 10 μm in (C–E); Scale bars = 20 μm in (F–I). Image published in: Li B et al. (2016) Copyright © 2017 Li, Qiu, Jayakannan, Xu, Li, Mayo, Tester, Gilliham and Roy. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |