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Figure 7. NPF2.5 amiRNA lines treated with 75 mM NaCl. Four-week old hydroponically grown T2 NPF2.5 amiRNA lines were treated with 75 mM NaCl for 5 days before harvest. (A) NPF2.5 transcript abundance detected in roots of knockdown lines and null segregate controls. (B) Shoot Cl− accumulation in knockdown lines and null segregate controls. (C) Shoot NO3− accumulation in knockdown lines and null segregate controls. (D) Shoot Na+ accumulation in knockdown lines and null segregate controls. (E) Shoot K+ accumulation in knockdown lines and null segregate controls. Results are presented as mean ± SEM (n = 4); significance is indicated by asterisks (one way ANOVA and Tukey test, *P ≤ 0.05; **P ≤ 0.01). Image published in: Li B et al. (2016) Copyright © 2017 Li, Qiu, Jayakannan, Xu, Li, Mayo, Tester, Gilliham and Roy. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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