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Fig 2. Multi-exponential fitting of compound outward current decay in male myocytes with different KChIP2 genotypes.Outward currents were activated by 5 s voltage pulses from -80 to +40 mV in male KChIP2+/+, KChIP2+/- and KChIP2-/- myocytes. The voltage protocol is shown below the current traces. A. Representative current traces for the different KChIP2 genotypes were normalized to peak and only the inactivating current components are shown (dotted line represents non-inactivating current level). Current decay kinetics in KChIP2+/+ (grey), KChIP2+/- (orange) and most KChIP2-/- myocytes (19 out of 26, blue, fast decay) were best described by a triple-exponential function. In some KChIP2-/- myocytes (7 out of 26, blue, slow decay) a double-exponential function was sufficient. B. Mean time constants (τ1, τ2 and τ3) obtained with a triple-exponential function for male KChIP2+/+ (grey bars), KChIP2+/- (orange bars) and most KChIP2-/- myocytes (19 out of 26, blue bars), and mean time constants obtained with a double-exponential function for 7 out of 26 male KChIP2-/- myocytes (τ2 and τ3, separate blue bars on the right). C. Mean amplitudes of the individual time constants obtained by triple-exponential (A1, A2 and A3) and double-exponential fitting (A2 and A3, separate bars on the right), and mean amplitudes of the corresponding non-inactivating current components (A0, A0). Note the KChIP2 gene dosage effect on A1. D. Mean total amplitudes of the compound outward current (AΣ, AΣ). The KChIP2 gene dosage effect observed for A1 is also reflected in AΣ (* significantly different from KChIP2+/+; ** significantly different from both KChIP2+/+ and KChIP2+/-; one-way ANOVA). Image published in: Waldschmidt L et al. (2017) © 2017 Waldschmidt et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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