XB-IMG-155617
Xenbase Image ID: 155617
|
Fig 3. Recovery from inactivation in male myocytes with different KChIP2 genotypes.Recovery from inactivation at -80 mV was studied in male myocytes using a double-pulse protocol with interpulse durations between 6 ms and 6.14 s (Δt) followed by a brief test pulse (voltage protocol shown below current traces). A. Representative current families, recorded with the double-pulse protocol for the different KChIP2 genotypes (dotted lines represent zero current). B. Relative peak current amplitudes obtained with the brief test pulse were plotted against interpulse duration for the different KChIP2 genotypes (KChIP2+/+: grey, KChIP2+/-: orange, KChIP2-/-: blue), and the data were fitted with a double-exponential function. Recovery kinetics for KChIP2+/+ and KChIP2+/- were virtually identical. Lines without symbols represent single-exponential functions fitted to a fraction of the data points and forced to reach 1 (orange and grey line superimpose). They represent the fast recovery component and emphasize the necessity of a double-exponential function to adequately fit the recovery kinetics for all KChIP2 genotypes. C. Mean recovery time constants (τrec1 and τrec2) obtained by double-exponential fitting (** significantly different from both KChIP2+/+ and KChIP2+/-; one-way ANOVA). D. Mean relative amplitudes of the recovery time constants (Arec1 and Arec2). Image published in: Waldschmidt L et al. (2017) © 2017 Waldschmidt et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |