Xenbase Image ID: 155655

Figure 1. KChIP2b differentially augments Kv4.3L and Kv4.3S current magnitude. (A) Exemplar current traces recorded at +40 mV (voltage protocol inset) from Xenopus oocytes 36–40 h after injection of 0.2 ng cRNA encoding Kv4.3L or Kv4.3S, with or without 1 ng KChIP2b (n = 14–18). Insets: left, scale bars; right, Voltage clamp protocol. Zero current level indicated by dashed line. (B) Mean ± SEM peak current magnitude at +40 mV for currents recorded as in (A) but with 0.04–5 ng Kv4.3L or S cRNA, with or without 1 or 5 ng KChIP2b cRNA, injected per oocytes, as indicated (n = 14–37). **P < 0.01 vs. all other groups with similar quantity of cRNA injected, by ANOVA followed by Tukey's HSD test. (C) Sequence alignment (Clustal TCoffee) of human Kv4.2, Kv4.3L and Kv4.3S protein sequences in the S6 to C-terminal region, including the segment missing in Kv4.3s (underlined red). (D) Exemplar current traces recorded at +40 mV (voltage protocol as in panel A) from Xenopus oocytes 36–40 h after injection of 1 ng cRNA encoding hKv4.2 with or without 5 ng hKChIP2b cRNA (n = 16–18). Scale bar inset. Zero current level indicated by dashed line. (E) Mean ± SEM peak current magnitude at +40 mV for currents recorded as in (E) (n = 16–18). ***P < 0.0001 between groups. (F) Comparison of Kv4.x current augmentation by hKChIP2b, analyzed from data in panels B and F (1 ng Kv4.x, 5 ng hKChP2b cRNA; n = 16–37). Image published in: Abbott GW (2017) Copyright © 2017 Abbott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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