Xenbase Image ID: 155656

Figure 2. KChIP2b differentially affects Kv4.3L and Kv4.3S inactivation rate and voltage dependence. (A) Exemplar current traces recorded from Xenopus oocytes 36–48 h after injection of 1 ng cRNA (as indicated) encoding Kv4.3L or Kv4.3S alone (black, n = 13–14) or in addition to 5 ng cRNA encoding KChIP2 (red, n = 13–31). Insets: right, Voltage clamp protocol; left, scale bars. Zero current level indicated by dashed line. (B) Mean ± SEM peak current/voltage relationship for currents as in (A); n-values as in (A). (C) Box plots showing individual and mean ± SEM values for fast and slow τ of inactivation, and relative amplitude of the fast component (double exponential fit), of Kv4.3L vs. Kv4.3S, recorded as in (A). n = 13–14. NS, P > 0.05. (D) Box plots showing individual and mean ± SEM values for τ of inactivation (single exponential fit) of Kv4.3L vs. Kv4.3S, with KChIP2 co-expression. Currents recorded as in (A); n = 13–14. ***P < 1 × 10−15. (E) Upper, exemplar current trace recorded using a steady-state inactivation protocol, from Xenopus oocytes 36–48 h after injection of cRNA encoding Kv4.3L (1 ng) and KChIP2 (5 ng). Insets: left, Voltage clamp protocol; right, scale bars. Zero current level indicated by dashed line. Lower, mean ± SEM fraction of available channels/voltage relationship for Kv4.3L vs. Kv4.3S, with/without KChIP2b; currents recorded as in (E); n = 6–8. (F) Upper, exemplar current trace recorded using an inactivation recovery protocol, from Xenopus oocytes 48 h after injection of cRNA encoding Kv4.3L (1 ng) and KChIP2 (5 ng). Insets: left and lower right, scale bars; center, voltage clamp protocol. Zero current level indicated by dashed line. Lower, mean ± SEM fractional recovery from inactivation/recovery time for Kv4.3L vs. Kv4.3S, with/without KChIP2b; recorded as in protocol above; n = 4–6. Image published in: Abbott GW (2017) Copyright © 2017 Abbott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

Return to previous page