Xenbase Image ID: 155657

Figure 3. KCNE1 differentially affects Kv4.3L and Kv4.3S function with/without KChIP2b. (A) Mean ± SEM peak current/voltage relationship for current traces recorded from Xenopus oocytes 48 h after injection of cRNA encoding Kv4.3L or Kv4.3S (1 ng) with KCNE1 (5 ng), with or without KChIP2b (5 ng; n = 9–17). Inset: Voltage clamp protocol. **P < 0.01 compared to other currents at +40 mV. (B) Box plots showing individual and mean ± SEM values for τ of fast and slow components, and relative amplitude, of fast inactivation (double exponential fit) of Kv4.3L-KCNE1 vs. Kv4.3S-KCNE1 currents recorded as in (A); n = 7–11. *P < 0.05; **P < 0.01; NS, P > 0.05. (C) Box plot showing individual and mean ± SEM values for τ of Kv4.3L-KCNE1-KChIP2b vs. Kv4.3S-KCNE1-KChIP2b inactivation (single exponential fit) for currents recorded as in (B); n = 8–17. **P < 0.01. (D) Mean ± SEM fraction of available channels/voltage relationship for Kv4.3L-KCNE1-KChIP2b vs. Kv4.3S-KCNE1-KChIP2b currents; voltage protocol upper right inset; n = 6. (E) Mean ± SEM fractional recovery from inactivation/recovery time for Kv4.3L-KCNE1 vs. Kv4.3S-KCNE1 with (n = 6) or without (n = 3–4) KChIP2b; voltage protocol upper right inset. Image published in: Abbott GW (2017) Copyright © 2017 Abbott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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