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Figure 5. KCNE3 differentially affects Kv4.3L and Kv4.3S current magnitude with KChIP2b. (A) Mean ± SEM peak current/voltage relationship for current traces recorded from Xenopus oocytes 36–48 h after injection of cRNA encoding Kv4.3L or Kv4.3S (1 ng) with KCNE3 (5 ng) with or without KChIP2 (5 ng; n = 11–20). Inset: Voltage clamp protocol. ***P < 0.001 compared to other currents at +40 mV. (B) Box plots showing individual and mean ± SEM values for τ of fast and slow components, and relative amplitude, of fast inactivation (double exponential fit) of Kv4.3L-KCNE3 vs. Kv4.3S-KCNE3 currents recorded as in (A); n = 10–13. NS, P > 0.05. (C) Box plot showing individual and mean ± SEM values for τ of Kv4.3L-KCNE3-KChIP2 vs. Kv4.3S-KCNE3-KChIP2 inactivation (single exponential fit) for currents recorded as in (B); n = 11–15. NS, P > 0.05. (D) Mean ± SEM fraction of available channels/voltage relationship for Kv4.3L-KCNE3 vs. Kv4.3S-KCNE3 with/without KChIP2; voltage protocol upper right inset; n = 5–7. (E) Mean ± SEM fractional recovery from inactivation/recovery time for Kv4.3L-KCNE3-KChIP2 vs. Kv4.3S-KCNE3-KChIP2; voltage protocol upper inset; n = 4–7. Image published in: Abbott GW (2017) Copyright © 2017 Abbott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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