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Xenbase Image ID: 155660
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Figure 6. KCNE4 differentially affects Kv4.3L and Kv4.3S function with KChIP2b. (A) Mean ± SEM peak current/voltage relationship for current traces recorded from Xenopus oocytes 36–48 h after injection of cRNA encoding Kv4.3L (filled squares) and (circles)/or (open squares) Kv4.3S (1 ng), with KCNE4 (5 ng), with/without KChIP2 (5 ng; n = 10–17). Lower right inset: Voltage clamp protocol. **P < 0.01 compared to all other currents at +40 mV, by one-way ANOVA with post-hoc Tukey HSD-test. Upper right inset: no effect on current density of individually increasing Kv4.3L (red columns) or Kv4.3S (open columns) cRNA injected, from 1 to 5 ng per oocyte, when co-injected with KCNE4S and KChIP2b cRNA (n = 11–17). NS = P > 0.05 by one-way ANOVA with post-hoc Tukey HSD-test; all other group comparisons P < 0.01. (B) Box plot showing individual and mean ± SEM values for τ of inactivation (single exponential fit) for Kv4.3L and / or Kv4.3S co-expressed with KCNE4 and KChIP2; n = 7–15. ***P < 5 × 10−6 vs. other groups; other comparisons P > 0.05. (C) Mean ± SEM fraction of available channels/voltage relationship for Kv4.3L and/or Kv4.3S co-expressed with KCNE4 and KChIP2; voltage protocol upper right inset; n = 5–6. (D) Mean ± SEM fractional recovery from inactivation/recovery time for Kv4.3L and/or Kv4.3S co-expressed with KCNE4 and KChIP2; voltage protocol upper inset; n = 5–6. Arrow, overshoot following recovery from inactivation. Image published in: Abbott GW (2017) Copyright © 2017 Abbott. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |