Xenbase Image ID: 155691

Figure 2. Uch37 physically interacts with Tcf7 in nucleus.(a) Co-IP assay in HEK293FT cells. Cells were transfected with indicated plasmids. myc-ADRM1 is known as an interacting partner of Uch37 and used as a positive control11. 48 h after transfection, cell lysates were precipitated with anti-myc antibody. (b) GST pulldown assay using purified proteins. His-Uch37 and GST-Tcf7 proteins were incubated and then subjected to immunoblotting. (c) Co-IP assay using nuclear extract of Xenopus gastrula embryo. One-cell stage embryos were injected with myc-Tcf7 (500 pg) and HA-Uch37 (500 pg) and cultured until stage 11, and then subjected to fractionation. Nuclear extracts were subjected to Co-IP assay using anti-myc or anti-HA antibody. (d) Co-IP assay using HEK293FT cells, HA-Uch37 and indicated myc-tagged truncated mutants of Tcf7 were transfected. Cell lysates were immunoprecipitated with anti-myc antibody. Truncated mutants of Tcf7 are depicted on the left. Tcf7, a full length; Tcf7 1–196, amino acids 1–196; Tcf7 240–365, amino acids 240–365; Tcf7 52–239, amino acids 52–239. Full images of all Fig. 2 are presented in Supplementary information (Fig. S9). Image published in: Han W et al. (2017) Copyright © 2017, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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