XB-IMG-155696
Xenbase Image ID: 155696
|
Fig. 1. Recombinant
plasmids. (A,B) Constructs encoding the shuttle proteins OS4 (A) and OS6 (B). LIG, vitellogenin ligand; CKP, casein kinase phosphorylation site; NLS, SV40 nuclear localization signal; RED, red fluorescent protein (in OS4 only); STREP, streptavidin core; 6HIS, His tag. The different segments were either cut from corresponding plasmids, synthetic oligonucleotides, or synthesized by PCR, adding appropriate linkers. For sequences see Fig. S1 (OS4) and Fig. S2 (OS6). (C) Donor plasmid Reco-σ with unique 5′ segment (5′), internal repeat (R) and unique 3′ segment (3′). AmpR and KanaR, genes coding for ampicillin and kanamycin resistance respectively. (D) Plasmid pEGFP-σ contains two 320 bp repeats of the coding segment of pEGFP-C1 between the BclI and GsuI sites, separated by a linker (S) with AccI and ApaI restriction sites. 5′, R, 3′, as in C. (E) Intramolecular homologous recombination within linearized Reco-σ and EGFP-σ DNAs. Crossing-over (×) takes place between the homologous repeats (R). Image published in: Rungger D et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |