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Fig. 2. DNA binding and nuclear translocation. (A) Gel retardation by commercial streptavidin and OS6 protein (arrow) of an oligonucleotide that was biotinylated at the 5′ end of one strand and carried the fluorescent dye Cy5 on the 5′ end of the other. The numbers on the abscissa correspond to nominal excess of streptavidin or OS6 molecules if the extracts were 100% pure. From this titration the amount needed to bind a given number of donor DNA molecules was calculated. (B) After injection of pEGFP-C1 into albino oocytes, the green fluorescence expressed from this plasmid remains in the cytoplasm. (C) The red fluorescent shuttle protein, produced from a plasmid expressing OS4 under the control of a CMV promoter, migrates to the oocyte nucleus. Image published in: Rungger D et al. (2017) © 2017. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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