XB-IMG-155704
Xenbase Image ID: 155704
Figure 1. The kinase Mps1 and its role in mitotic checkpoint signaling.(A) Mps1 phosphorylates (P) three different proteins to promote the assembly of the mitotic checkpoint complex. It phosphorylates the kinetochore protein KNL1 to recruit the checkpoint protein complex Bub1-Bub3 to KNL1 (1). It phosphorylates Bub1, which allows this protein to interact with another checkpoint protein, Mad1 (2). It also phosphorylates Mad1, which promotes the binding of Mad2 to the regulatory protein Cdc20 (3). Ji et al. propose that phosphorylated Mad1 binds to Cdc20, thereby positioning the latter for capture by Mad2. (B) The checkpoint (represented by the STOP sign) is only active when Mps1 has phosphorylated all three proteins, KNL1, Bub1, and Mad1. (C) Checkpoint activity (y-axis) plotted as a function of Mps1 kinase activity (x-axis) for the phosphorylation of one (P), two (PP) or all three sites (PPP). Image published in: Ciliberto A and Hauf S (2017) © 2017, Ciliberto et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View |