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XB-IMG-155705

Xenbase Image ID: 155705


Figure 1. Gsc-mediated CE phenotypes in Xenopus.(A) Experimental design. Specimens were injected with Gsc-GR into the dorsal marginal region of the 4-cell embryo and cultured to the stages indicated, with or without addition of dex. (B–E) Gsc-GR induced NTD and BPD in whole embryos. Specimens were scored for wt appearance (blue; B), NTD (green; C) and BPD (red; D). Anterior is to the left in (B–D). (E) Compilation of results. Note that Gsc-GR caused CE phenotypes in a highly significant proportion of embryos, but only when activated before and during gastrulation. Note also that deletion of the homeodomain (∆HD) or altering the DNA-binding specificity (K197E) prevented BPD/NTD-induction, while the repression domain GEH was not required for BPD/NTD. (F–I) Impaired CE of the notochord upon sustained dorsal Gsc-GR expression. Note that the notochord was wider and shorter in dex-treated (G,I) as opposed to untreated (F,H) specimens, both at stage 14 (F,G) and stage 19 (H,I). (J,K) Repression of Xbra transcription on the dorsal side upon Gsc-GR activation. (L,M) Double axis formation (M) following ventral injections of Dgsc mRNA into 4-cell Xenopus embryos (L).

Image published in: Ulmer B et al. (2017)

Image downloaded from an Open Access article in PubMed Central. Copyright © 2017, The Author(s)

GeneCloneSynonymsSpeciesStage(s)Tissue
tbxt.L Xbra, X-bra, bra, Xbrachyury, brachyury, ntl, t-a, t-b, tX.laevisThroughout NF stage 14circumblastoporal collar
tbxt.L Xbra, X-bra, bra, Xbrachyury, brachyury, ntl, t-a, t-b, tX.laevisThroughout NF stage 19notochord
circumblastoporal collar
mesoderm
tbxt.L Xbra, X-bra, bra, Xbrachyury, brachyury, ntl, t-a, t-b, tX.laevisThroughout NF stage 11mesoderm

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