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Figure 5. Gsc-GR inhibits membrane recruitment of Dvl2.(A) Co-injection of mRNAs as indicated into the animal region of all cells at the 4-cell stage or of selected cells at the 8-cell stage. Embryos were cultured ± dex (added at st. 6/7), animal cap tissues were excised at stage 10 and subjected to live imaging. (B–E) Membrane localization of Dvl2-GFP was significantly impaired upon Gsc-GR activation. (B–D) Examples of specimens from the same batch of embryos and photographed with the same exposure times showing lack of localization (B; red), good (C; green) and attenuated localization (D; blue). (E) Quantification of results (p = 0.002). (F,G) Cell-autonomous effect of Gsc-GR. Injection of Gsc-GR in 1/4 animal cap cells at the 8-cell stage (cf. A) resulted in attenuation of Dvl2-GFP membrane recruitment upon dex treatment (cf. F’ and G’). *mark Gsc-GR-injected cells, as revealed by fluorescence of lineage tracer mRFP. Image published in: Ulmer B et al. (2017) Copyright © 2017, The Author(s). This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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