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Xenbase Image ID: 155710

Figure 6. Rescue of Gsc-GR mediated NTD/BPD by Wnt/PCP pathway components.Xenopus embryos were injected with the indicated mRNAs into the dorsal marginal region of all cells at the 4-cell and cultured to stage 22. Dex was added when Gsc-GR was used. Specimens were scored for normal appearance (blue bars), NTD (green) and BPD (red). (A) constitutively active RhoA; (ca; A) dominant-negative (dn) RhoA; (C) Prickle; (D) Vangl2; (E) Brachyury; (F) Wnt11. Uninjected embryos (uninj.) served as controls. Note that rescue was observed upon co-injection of Gsc-GR with ca-RhoA, Prickle, Vangl2, Brachyury and Wnt11, while enhanced phenotypes were seen with co-injected dn-RhoA. As embryos in the latter combination showed high rates of lethality, the dose of injected Gsc-GR was reduced from 400 pg to 160 pg. Cf. Table S1 for numbers and statistics.

Image published in: Ulmer B et al. (2017)

Image downloaded from an Open Access article in PubMed Central. Copyright © 2017, The Author(s)

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