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Figure 4—figure supplement 2. In planta characterization of the indole-specific glucosinolate transporter GTR3 – aliphatic glucosinolate data.Aliphatic glucosinolate concentration of roots (A) and rosettes (B) of 3-week-old hydroponically grown A. thaliana wildtype (Col-0), gtr3, gtr1 gtr2 and gtr1 gtr2 gtr3 plants. For individual glucosinolate data see Figure 4—source data 1–2. The box is determined by the 25th and 75th percentiles. The whiskers are determined by the 5th and 95th percentiles. Median and mean are shown as line and square. Groups in subfigures are determined by one-way ANOVA followed by Tukey HSD Calculator multiple comparison post-hoc analysis (p<0.05). Data presented is one of two individual experiments, each containing 8–12 repeats (n) (see Figure 4—source data 1–2 for individual glucosinolate data points and individual n; error bars and parentheses are s.d. Post-hoc Tukey HSD Calculator multiple comparison (p<0.05). n.d. none detected. (C) Aliphatic glucosinolate concentration of rosettes and roots of 3-week-old hydroponically grown reciprocally micro-grafted wild type (wt), myb28 myb29 cyp79b2 cyp79b3 glucosinolate biosynthesis null mutant (qko) and gtr1 gtr2 gtr3 (tko). Rosettes and roots from wild type wt, qko and tko plants were reciprocally grafted using 4-day-old seedlings. The grafts are shown as the genotype of rosette and roots, respectively. Aliphatic glucosinolate content in the rosette and roots was quantified by LC-MS in 3-week-old plants. Data presented is one of two individual experiments, each containing 8–16 repeats (n) (see Figure 4—source data 3–4 for individual glucosinolate data points and individual n; error bars and parentheses are s.d. Groups in subfigures are determined by one-way ANOVA followed by Tukey HSD Calculator multiple comparison post-hoc analysis (p<0.05). nd none detected.DOI: http://dx.doi.org/10.7554/eLife.19466.015 Image published in: Jørgensen ME et al. (2017) © 2017, Jørgensen et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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