Xenbase Image ID: 155796

Fig 4. Increased cardiomyocyte size persists eleven months post amputation. (A) Using the WGA labelled heart pictures, the cell-membrane labelled boundaries of orthogonal view cardiomyocytes (left panel) was delineated in red (middle panel) and filled with blue colour (right panel) for non-amputated control hearts (CTRL) and different time-points post-amputation (7 dpa, 1, 3, 6 and 11 mpa). (B) The cross-sectional area of each cell was automatically calculated using ImageJ software. Quantification was performed on 2 or 3 independent heart sections, corresponding to minima of 300 cell areas counted for each group, and samples were compared to the CTRL. Animals: CTRL, n = 1; 7dpa, n = 1; 1mpa, n = 1; 3mpa, n = 1; 6mpa, n = 1, 11mpa, n = 1. An unpaired non-parametric t-test (Mann Whitney) was performed: ****, p<0.0001. Image published in: Marshall L et al. (2017) © 2017 Marshall et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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