XB-IMG-155797
Xenbase Image ID: 155797
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Fig 5. Long lasting cardiac hypertrophy occurs in amputated frog heart.
Cardiomyocytes were observed at the site of amputation (A, D, G, J, M, P) and in a remote zone of the amputated ventricle (B, E, H, K, N, Q), using immuno-detection of natriuretic peptide A as an indicator of cardiac hypertrophy. Sections were labelled for tropomyosin (CH1, red), natriuretic peptide A (NPPA, green) and DAPI-counterstained nuclei (blue) at different times-points after amputation (7 dpa and 1, 3, 6 & 11 mpa) and compared with a control non-amputated heart (CTRL). In the vicinity of the amputation site, a weak NPPA signal was detected in CTRL (A), slightly increased at 7 dpa (D), whereas a clear increase of NPPA labelling in the myocardium was evident from 1 mpa to 11 mpa (G, J, M, P). In the remote zone, the NPPA labelling seemed appeared to slightly increase over time compared to the CTRL (compare B, E, H, K, N, Q). As a labelling control, all samples displayed an intense NPPA signal in atrium (C, F, I, L, O, R), since nppa is highly expressed in this tissue. Animals, CTRL, n = 1; 7dpa, n = 1; 1mpa, n = 1; 3mpa, n = 1; 6mpa, n = 1, 11mpa, n = 1. Scale bars, 200 μm (A–R). Image published in: Marshall L et al. (2017) © 2017 Marshall et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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