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Fig 7. Short-term transcriptional responses in adult frog heart after cardiac injury display evidence of a scarring process. (A) Heart ventricles from different experimental conditions were collected for RT-qPCR analysis: control non-operated hearts (CTRL), SHAM-operated hearts where only the pericardium was opened (scissor 1, SHAM), and amputated hearts where a ventricle biopsy was performed following pericardium opening (scissor 1 & 2, AMP). (B) RNA extraction was performed on whole heart ventricles, collected at days 1, 3 and 7 post-amputation followed by RT qPCR to quantify gene expression. Gene markers of fibrosis (fn1, col1a1, ctgf), hypertrophy (odc, nppa, nppb), cellular structure (actn3, tnnt2), proliferation (pcna, ccnd1, tert), inflammation (cebpb, il1b, cxcl8), actl6a and akt1 were monitored. Aligned dot plot with median and interquartile range, n≥4 for each group with exact n displayed above the x-axis. Normalisation was performed using the geometric mean of two reference genes (smarcd1/smn2), fold-change is shown in log2 scale, respective to SHAM for each gene at each individual time-point. *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001. Image published in: Marshall L et al. (2017) © 2017 Marshall et al. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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