XB-IMG-155823
Xenbase Image ID: 155823
|
FIGURE 2. Akirin2 knockdown disrupts neural development in Xenopus. (A-C) Morphology of
tadpoles (stage 37/38) injected with standard MO (25 ng), Akirin2 MO (25 ng), Akirin2 MO (25 ng) and
Akirin2 mRNA (0.6 ng). Embryos were injected in both blastomeres at the two-cell stage and raised to
tadpole stage. (D-I) Akirin2 MO (25 ng) with or without Akirin2 mRNA (0.6 ng) was injected into one
cell of four-cell-stage embryos, and whole-mount in situ hybridization with probes of Sox2, Nkx6.2 and
N-tubulin was processed at St.14-16. LacZ mRNA was co-injected to trace the injected sides (stained red
on the left sides). (J) Quantification of the effects of the injection of Akirin2 MO or co-injection of
Akirin2 MO and Akirin2 mRNA on the expression of Sox2, Nkx6.2 and N-tubulin as shown in (D-I) Image published in: Liu X et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |