XB-IMG-155826
Xenbase Image ID: 155826
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FIGURE 5. Akirin2 and Geminin antagonistically regulate Sox2 expression. (A, B) Co-IP assays of
exogenous Akirin2 and Geminin proteins in HEK293 cells. The cells were transfected with the indicated
plasmids, and the cell extracts were immunoprecipitated and detected with the indicated antibodies. (C)
Interactions of various Akirin1-Akirin2 fusion constructs with Geminin in co-IP experiments. (D) A
schematic map of the Geminin truncations with amino acid numbers indicated. (E) Interactions of various
Geminin truncation constructs with Akirin2 in co-IP experiments. (F) BAF53a enhances the interaction of Akirin2 and Geminin. (G-K) Co-injection with either Akirin2 or BAF53a mRNA rescues the expansion of
Sox2 in the Geminin misexpression embryos, whereas this effect was reversed by co-injection of BAF53a
MO or Akirin2 MO. LacZ mRNA was co-injected to trace the injected sides (stained red on the left sides).
(L) Quantification of the effect of the injection of mRNA or co-injection of MO as shown in (G-J). WCL,
whole cell lysate; IB, immunoblot; IP, immunoprecipitation; FL, full-length. Image published in: Liu X et al. (2017) Copyright © 2017. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View |