XB-IMG-155843
Xenbase Image ID: 155843
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Zebrafish islet1 enhancer predominantly labels Rohon Beard neurons and evx1 enhancer labels V0v neurons in Stage 41 Xenopus laevis spinal cord. (A–C) DAB immunohistochemistry for EGFP in transient Tg(islet1:cfos:Gal4VP16;UAS:EGFP) transgenic spinal cord. (A) Dorsal view of spinal cord, rostral left showing labeled RB neuron soma (*) and longitudinal axons in the dorsal tract (black arrows). The smaller, more weakly labeled cells are probably not RB neurons. (B and C) lateral views, rostral left, dorsal top. (B) RB neuron with characteristic large round soma in dorsal spinal cord (*) and ascending and descending longitudinal axons (black arrows), + indicates a pigment cell located ventral and lateral to the RB neuron. (C) shows a slightly more ventrally located neuron in the same embryo as (B) with an oval soma and ventral axon (arrows). (D) Lateral view with rostral left and dorsal top showing DAB immunohistochemistry for EGFP in transient Tg(evx1:cfos:Gal4VP16;UAS;EGFP) transgenic spinal cord. Labeled cells have pear-shaped soma, axons that extend ventrally and then become commissural and are located in the dorsal 48–68% of the spinal cord. White crosses indicate dorsal pigment cells. (E) Fluorescent immunohistochemistry for EGFP in transient Tg(evx1:cfos:Gal4VP16;UAS:EGFP) spinal cord; cross section (dorsal up) showing a single labeled neuron soma and ventral commissural axon. White dotted lines show edge of spinal cord. (F–H and J–L) Double labels (in situ hybridization with BM purple plus fluorescent immunohistochemistry for EGFP) of Tg(evx1:cfos:Gal4VP16;UAS:EGFP) spinal cord cross sections. Spinal cord margins are delinated with dotted lines. (F) X. laevis evx1 RNA expression (purple). (G) EGFP expression (green) in same cross section as (F). (H) merged image of F and G. Two EGFP-labeled cells (X) co-express evx1 RNA. (J) X. laevis slc17a7 RNA expression (purple). (K) EGFP expression (green) in same cross section as (J). (L) merged image of J and K. Two EGFP-labeled cells (X) coexpress slc17a7 RNA. (I) schematics showing approximate region of spinal cord shown in dorsal view in (A) and lateral views in (B–D). (B and C) show only part of this region. (A–L) are all Stage 41. Scale bar in A = 20 μm (panels A–H and J–L) and scale bar in I = 1 mm. Image published in: Juárez-Morales JL et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons. Larger Image Printer Friendly View |