XB-IMG-155912
Xenbase Image ID: 155912
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Fig. 1. Mid1 is expressed in cells of the forming optic stalk and induced on shh RNA injection. (A) mid1 mRNA expression was assessed by Wmish on Xenopus laevis embryos at different NF stages. (a–d) Lateral view, (a′) cranial view, and (b′–d′) lateral view. Twenty-micrometer gelatin/albumin sections at the level of the tadpole’s head (d1–d3), heart (c1), neural tube (c2), and pronephric anlage (c3). cg, cement gland; ec, endocardium; ed, eye disk; ev, eye vesicle; hd, hindbrain; he, heart; hf, heart field; kt, kidney tubules; mb, midbrain; mc, myocardium; op, olfactory placode; os, optic stalk; ov, otic vesicle; pnt, pronephric tubule; pos, presumptive os; sm, somites. (c4–c7) mid1 expression in eye stalk region in serial sections. (B) Injections were done into one cell of two-cell stage embryos. (a–d′) Synthetic shh-RNA (500 pg) was injected and Wmish against mid1, pax2, pax6, and ptc1 was performed as indicated. (e–f′) pax2-mo (2.5 pmol) was injected, and expression of mid1 and pax6 was monitored. Shown are transversal sections of the eye at the level of the lens. (g–g′) pax6-mo (2.5 pmol) was injected, and pax2 was monitored. Shown are lateral views of the head region and a transversal section of the head region at the level of the optic vesicles (g′). (h–h′) pax2 RNA (100 pg) was injected and expression of pax6 was analyzed. Image published in: Pfirrmann T et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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