XB-IMG-155917
Xenbase Image ID: 155917
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Fig. 4. Mid1 loss of function interferes with eye development. (A and B) 2.5 pmol of mid1-mo1 was injected into one cell at the two-cell stage and analyzed by Wmish at NF stage 38. (Upper) Lateral views of head region of noninjected (a; NIS) or injected side (a′; IS), dorsal view (a′). (B) Analysis of retinal stratification on mid1 suppression in transversal sections at the level of the lens using probes against pax6, brn3.0, vsx1, prox1, and rhodopsin. For better comparison, all images are oriented with the lens to the left. (C) Lateral view (a and b) of NF stage 38 embryos injected with mid1-mo2 into one cell at the two-cell stage and 10× enlarged view of the eye region of a′ and b′ (Lower). All mid1-mo2–injected embryos showed an increase in eye size (n = 212 mid1-mo2 and n = 126 ctrl-mo–injected embryos). The graph shows the mean values for 25 embryos (c; **P = 0.001). Analysis of cell proliferation of mid1-mo2–injected embryos. The number of phospho-histone H3 (pH3) positive cells were counted and compared. (d; ctrl-mo: six embryos, mean 14.4 pH3+ cells per section; mid1-mo2: eight embryos, mean 27.2 pH3+ cells per section; ***P = 0.0006). (D) Pax6 immunoreactivity in cryosections on mid1-mo1 injection. The number of Pax6-positive cells per sections within the retinal region was counted for both sides of two embryos, and the area of the total retina was estimated. The numbers of Pax6-positive cells from sections of three mid1-mo1–injected embryos were counted (c; *P = 0.03); the numbers of Pax6-positive cells relative to the area of the retina is shown in the right diagram (d). The value for the noninjected side was set to 1. Image published in: Pfirrmann T et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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