Xenbase Image ID: 155918

Fig. S3. (A) a1–a4 show a series of transversal sections at the level of the eye of the mid1 injected tadpole (NF stage 38) shown in Fig. 1 A, a. (B) Rescue of the mid1 knockdown eye phenotype. Lateral view of NF stage 38 embryos injected with mid1-mo2 (1.25 pmol) along with synthetic human mid1 RNA (1–2 ng) into one cell of a 2-cell stage embryo. The lower panel shows a 10× enlarged view of the respective eye region. Considering the eye to be an oblate spheroid, the mean radius was estimated by measuring the drawn distances and total volume was calculated. The graph shows the mean values for 20 embryos. The specificity of the pax2 and pax6 morpholino had been tested already by rescue experiments in refs. 53 and 54, respectively. Image published in: Pfirrmann T et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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