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Fig. 3. N4bp3 function is required for brain development. A Injection of 25 ng N4bp3 MO results in a reduced brain size (red dotted line, middle panel) compared to the un-injected and Control MO injected sides (black dotted lines, left panel). The brain phenotype was significantly rescued by co-injection of 25 ng N4bp3 MO together with 1 ng full-length rat N4bp3 RNA (right panel). Scale bar: 200 µm. B Quantification of the data in A. For the evaluation, the injected zone was set in ratio to the un-injected zone. Abbreviations: n, number of independent evaluated brains. Error bars indicate standard error of the means (s. e. m.); **** p≤0.0001. P-values were calculated by a nonparametric, one-tailed Mann-Whitney rank sum test. Image published in: Kiem LM et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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