Xenbase Image ID: 155935

Fig. 1. : Depletion of Wnt11a disrupts cardiogenesis in Xenopus laevis. A. Wnt11a MO injection leads to a cardiac phenotype characterized by cardiac edema (lateral view, red arrowheads) and smaller, deformed hearts (ventral view, dotted lines; isolated hearts) at stage 43. Scale bars: lateral view: 1000 μm; ventral view and isolated hearts: 250 μm. B. Quantitative presentation of the data shown in A. C. The heart rate is significantly reduced in Wnt11a-depleted embryos. D-G. Analysis of cardiac Troponin T stained Wnt11a morphant hearts using OPT. Atrial width, ventricular width and a-v length were measured as indicated in E resulting in data provided in F. Measurement of the OFT was done as indicated in E. Data are given in G. Scale bar: 100 μm. H. Cardiac Troponin T (Tnnt2) staining shows that Wnt11a inhibition results in smaller and malformed hearts. Cross sections demonstrate a disturbed ventricular trabeculation (t). Scale bars: 100 μm. White arrowheads indicate disturbed trabeculation. a, atrium; bpm, beats per minute; n, number of independent experiments; N, number of analysed embryos; ng, nanogram; oft, outflow tract; v, ventricle. Error bars indicate standard error of the means (s.e.m.). *, p≤0.05; ****, p≤0.0001; calculated by a non-parametric Mann-Whitney rank sum test. Image published in: Hempel A et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue tnnt2.L CT3, LOC446916 X. laevis Throughout NF stage 43 heart outflow tract cardiac ventricle Image source: Published Larger Image Printer Friendly View

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