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Supplement Fig. 4. Specificity and efficiency of Rcsd1 antisense morpholino oligonucleotides (MOs). A. 5’ untranslated region (UTR) of rcsd1 mRNA. Binding sites of Rcsd1 MOs 1–3 are indicated. The ATG start codon is highlighted in pink. B-C.In vivo Rcsd1 MO binding efficiency test. Rcsd1 MO-GFP-fusion constructs and MOs were co-injected bilaterally at 2-cell stage embryos as indicated. The Rcsd1 MOs specifically blocked the translation of the Rcsd1 MO-GFP fusion constructs (no gfp signal) but not the ∆5’UTR Rcsd1 MO-GFP fusion constructs (gfp signal). The Control MO does not bind to the Rcsd1 MO binding site. Image published in: Hempel A et al. (2017) Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V. Larger Image Printer Friendly View

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