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Fig. 1. Cryogenic preservation of X. laevis sperm is less effective and consistent than for X. tropicalis sperm. Testes from individual X. tropicalis (A) and X . laevis (B) were either used fresh or frozen and thawed using the Harland method to fertilise eggs from a single female of known good quality. A-I on the X-axis refers to sperm samples, each from a different male. The experiment was repeated using three female frogs, to eliminate this as a possible source of variability, data from a single female are shown. Eggs that divided were counted as fertilised, and morphologically normal embryos were counted at NF32. C) The mean values from the experiments above are shown for X. laevis and X. tropicalis ± SEM. There is a significant effect of the species on the percentage fertilisation (**: p = 0.002) and on the percentage of normal embryos at NF32 (***: p = 0.0003), whether fresh or frozen sperm was used. Image published in: Pearl E et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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