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Fig. 2. The Mansour and Harland methods are the most effective for long-term cryopreservation of X. laevis sperm. Sperm from 3 separate male frogs was either tested fresh on eggs from two females of known good quality or was tested following cryopreservation by the methods shown. Sperm were recovered after 2 days, 3 months or 13 months and fertilisation rates were recorded. Means ± SEM are shown. There is no significant difference between the Mansour and Harland methods (p = 0.82). There is a significant difference between the Harland and Sargent methods (*: p = 0.04) and between the Mansour and Sargent methods (*: p = 0.03). Image published in: Pearl E et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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