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Figure 5. Tranilast inhibits urate transport activity of URAT1 and GLUT9a in a noncompetitive (mixed) manner. (A) The kinetic curve of URAT1‐mediated [14C]‐urate uptake in the absence or presence of inhibitor (cold urate or tranilast). (B) The kinetic curve of GLUT9a‐mediated [14C]‐urate uptake in the absence or presence of inhibitor (cold urate or tranilast). (C) Eadie–Hofstee plot of URAT1‐mediated [14C]‐urate uptake in the absence or presence of inhibitor. (D) Eadie–Hofstee plot of GLUT9a‐mediated [14C]‐urate uptake in the absence or presence of inhibitor. V, the [14C]‐urate uptake rate in pmol/oocyte/h; V/S, [14C]‐urate uptake rate per concentration (μmol/L) of [14C]‐urate; open circle, in the absence of inhibitor; open circle, in the absence of inhibitor; open triangle, in the presence 50 μmol/L cold urate; closed circle 15 or 20 μmol/L tranilast; closed triangle 30 or 40 μmol/L tranilast. Tran, Benz, Prob, SalDMSO, W, oocytes were washed out of drugs with uptake medium. Data are mean ± S.E. with n = 12–15. Tran, Tranilast; Benz, Benzbromarone; Prob, probenecid; Sal, salicylate; DMSO, dimethylsulfoxide; W, oocytes were washed out of drugs with uptake medium. Image published in: Mandal AK et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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