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FIGURE 7. hRspo1 induces axis duplication and Xnr3 expression in the presence of LRP6 or Frizzled5 in Xenopus. A, hRspo1 induces axis duplication when co-injected with LRP6 or Frizzled5. mRNA was injected into the ventral marginal two cells at the 4-8-cell stage. The arrowhead indicates the location of the secondary axis. Note the complete axis duplication in the case of hRspo1 plus Frizzled5. B, summary of data from co-injection experiments. The white bar indicates the percentage of injected embryos that had trunk-tail type of secondary structures. The black bar indicates the percentage of injected embryos that had the secondary head structures. n, number of embryos injected/examined. un inj indicates not injected. C, Xnr3 was induced synergistically in the animal caps by co-injection of hRspo1 and LRP6 or Frizzled5. mRNA was injected into the animal pole of two-cell stage embryos, and the animal caps were dissected at stage 9.5 and cultured until stage 10.5 for RT-PCR analysis. PCR of samples without reverse transcription (RT-) was used as negative control showing no contamination of genomic DNA. The whole embryo (WE) was used as a positive control for RT-PCR. RT-PCR of EF-1α was used as a loading control. Image published in: Wei Q et al. (2007) Copyright © 2007. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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