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Figure 5. Recruitment of XPF‐ERCC1 mutants to the ICL during repair A. Schematic representation showing the primer locations on pICL and pQuant. B. pICL was replicated in XPF‐ERCC1‐depleted (ΔXE) or XPF‐ERCC1‐depleted egg extract supplemented with wild‐type (XEWT) or mutant XPF‐ERCC1 (XEMUT; see Appendix Fig S2). Samples were taken at various times and immunoprecipitated with α‐XPF antibodies. Co‐precipitated DNA was isolated and analyzed by quantitative PCR using the primers depicted in (A). The qPCR data were plotted as the percentage of peak value with the highest value within one experiment set to 100%. C–F As in (B) but using the indicated XPF‐ERCC1 mutant complexes. Experimental replicates are shown in Appendix Fig S2. Image published in: Klein Douwel D et al. (2017) © 2017 The Authors. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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