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Fig. 2. Gastrulation defects from over-expression of Xclaudin 1. (A) Injection of Xclaudin 1 mRNA 500 pg/embryo (n = 63) into designated blastomeres at 8-cell stage resulted in embryos with dorsally bent trunk and shorter or short anteroposterior axis in a dose-dependent manner. (B) Injection of Xclaudin 1 mRNA (500 pg/embryo) at four blastomeres of dorsal (n = 70), ventral (n = 65), animal (n = 68) or vegetal side (n = 65) of 8-cell stage embryos. The embryos were observed and analyzed at stage 23. (C) Morphology of embryos injected with various doses of Xclaudin 1 mRNA (250 pg/embryo (n = 58), 500 pg/embryo (n = 63) or 1 ng/embryo (n = 60)) and un-injected embryos (n = 67) was analyzed according to category of phenotypes. Numbers of embryos represents the percentage of embryos with normal morphology (open bar), a short (slanting line bar), shorter (gray solid bar), anteroposterior and dorsally curved trunk axis (black solid bar) out of total embryos examined. Image published in: Chang DJ et al. (2010) Copyright © 2010. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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