Xenbase Image ID: 156454

Fig. 2. Reduced Sgo1 recruitment to chromatin in the absence of CENP-C, CENP-T, Bub1, or Mps1. Immunoblot analysis of chromatin fractions from replicated chromosomes assembled in the indicated extracts and purified by centrifugation through a sucrose cushion (lanes 2–7). Chromatin purified in the same way from a mock assembly reaction without sperm served as control (no sp, lane 1). Histone H1 was used as loading control. Quantification of the Sgo1 signals, normalized to the H1 signals, and expressed relative to the Sgo1 signal in the chromatin obtained in the mock-depleted extract Image published in: Williams SJ et al. (2017) © The Author(s) 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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