Xenbase Image ID: 156455

Fig. 3. Mps1 is required for Bub1 and Sgo1 targeting to the centromere. a Immunoblot analysis of the extracts used in (b–e) to estimate the efficiency of the depletion and to confirm that these depletions did not alter Sgo1 levels in the soluble extracts. RbAp48 was used as loading control. b–e Representative examples of chromosomes assembled in extracts lacking Mps1 or Bub1, as well as in mock-depleted extracts, and stained with antibodies against Bub1 (b), Sgo1 (c), pH2A (d), or a Bub1 antibody (Bub1*) different from the one used in (b) and Fig. 1. CENP-C or CENP-A was used to label centromeres. Insets highlight an individual centromere pair within each chromosome mass. Scale bar, 10 μm Image published in: Williams SJ et al. (2017) © The Author(s) 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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