Xenbase Image ID: 156457

Fig. 5. Forced targeting of Bub1 to the centromere rescues Sgo1 targeting in the absence of kinetochores. a Schematic representation of the constructs used in (b–d). b Representative examples of chromosomes assembled in mock-depleted extracts and in CENP-C-depleted extracts supplemented with buffer, cenC, or cenBub1 and stained with the indicated antibodies. Insets highlight an individual centromere within each chromosome mass. Scale bar, 10 μm. c Quantification of the number of chromosome masses showing Sgo1 staining at centromeres in two different experiments (expressed as percentage). More than 120 centromeres were scored per condition in each experiment. d Chromosomes assembled as in (b) stained with pH2A and DAPI. Scale bar, 10 μm Image published in: Williams SJ et al. (2017) © The Author(s) 2016. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license Larger Image Printer Friendly View

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