Xenbase Image ID: 156468

Fig. 3. Panel A: RT-PCR of eEF1A1 in X. laevis muscle and liver using primers that amplify full-length mRNA. Lanes labelled with – are no-RT controls. Panel B: Real-time RT-PCR results for eEF1A1 and eEF1A2 in liver (showing expression of eEF1A1 only) and muscle (showing equal levels of expression of eEF1A1 and eEF1A2). Units of expression are relative to the internal control, Rpl8. Panel C: Analysis of expression of eEF1A1 in adult muscle from X. tropicalis. B shows RT-PCR of eEF1A1 and eEF1A2 in muscle from X. tropicalis (Xt) and X. laevis (Xl) together with negative controls (−RT). All PCR products are of the predicted size. Panel D: two Western blots, run in parallel, for eEF1A1 and eEF1A2. M(t) is muscle tissue from X. tropicalis, M(l) is muscle tissue from X. laevis and L(l) is liver from X. laevis. Image published in: Newbery HJ et al. (2011) Copyright © 2011. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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