XB-IMG-156482
Xenbase Image ID: 156482
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Fig. 2.
Overexpression of PlexinA1 leads to a reduction in migrating NC cells at neurula stages. (A) Xenopus embryos were injected with 500 pg PlexinA1 RNA and 100 pg lacZ RNA as a lineage tracer in one blastomere at the two-cell stage. Twist in situ hybridization was used to analyze NC migration at neurula stage 20 or tailbud stage 28. LacZ-injected control embryos are shown in the upper panel, PlexinA1 injected embryos in the lower panel. “+” marks the injected lacZ-positive (blue) side and “−” the uninjected side, black arrow head indicates NC migration defects. The left panels show an anterior view of a neurula stage embryo, the right panels the head region of injected tailbud stage embryos. (B) Graph summarizing seven independent injection experiments (three experiments did not include injections of 250 pg PlexinA1 RNA). Embryos were injected either with 100 pg lacZ RNA alone or in combination with 250 pg or 500 pg PlexinA1 RNA. Number of injected embryos is indicated for each column. (∗) NC migration defects were significantly different compared to lacZ injected controls (p < 0.01 in a student t-test) (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.). Image published in: Wagner G et al. (2010) Copyright © 2010. This image is reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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