Xenbase Image ID: 156489

Fig. 2. foxn4 Cas9/CRISPR phenotypes. (A-C) Representative confocal images of the skin in stage 26 wild-type control embryos (A) or embryos injected with Cas9 along with two independent gRNAs directed against foxn4 (B,C). Cell membranes (mRFP, blue), basal bodies (Centrin4-GFP, green) and cilia (acetylated tubulin, red) are labeled as in Fig. 1. (D,E) Representative confocal images of the skin of embryos at stage 26 or stage 30, following injection with Cas9 protein and Foxn4gRNA1. Cell boundaries (blue), basal bodies (green) and cilia (red) are labeled as above. (A-E) The percentage of MCCs (±s.d.) with defective cilia or undocked basal bodies is indicated based on scoring at least 12 fields from different embryos (n≥50 cells). (F) Scatter plot of basal body number located within 1 μm of the apical surface in MCCs of wild-type controls (blue, Cont) and Cas9/Foxn4gRNA1-injected embryos (red, G1) at the indicated stages. The central bar indicates the mean and error bars indicate s.d. ***P<0.001; n.s., not significant. Image published in: Campbell EP et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue tuba4b.L alpha tubulin, alpha-tubulin, tuba4, tuba4a X. laevis Throughout NF stage 26 cilium ciliated cell ciliated epidermal cell multiciliated cell Image source: Published Larger Image Printer Friendly View

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