XB-IMG-156490
Xenbase Image ID: 156490
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Fig. 3.
Similar functions of Foxj1 and Foxn4 downstream of Multicilin. (A,B) Representative confocal images of the skin of stage 30 wild-type control (A) or Cas9/Foxj1gRNA1-injected (B) embryos, where cell membranes (mRFP, blue), basal bodies (Centrin4-GFP, green) and cilia (acetylated tubulin, red) are labeled. The percentage of MCCs with cilia extension defects is indicated (±s.d.) based on scoring 12 fields from different embryos (n≥50 total cells). (C) The average number of MCCs, outer cells and putative ionocytes within a 0.085×0.085 mm field based on data from ≥12 embryos. Error bars indicate s.d. *P<0.05. (D) The percentage of MCCs extending >50, 10-50, 1-10 and 0 cilia in control and Cas9/Foxj1gRNA1-injected embryos based on scoring ≥147 cells from eight to ten embryos. Error bars indicate s.d. n.d., not detected. (E-G) Confocal images of the skin of wild-type (E), Cas9/Foxj1gRNA1-injected (F) or Cas9/Foxn4gRNA1-injected (G) embryos, further injected with RNAs encoding Multicilin-HGR, mRFP (blue) or Centrin4-GFP (green). At stage 11.5, Multicilin activity was induced by treatment with Dex, and embryos were fixed at stage 26 and stained for cilia (red). The percentage of MCCs with cilia extension defects is indicated (±s.d.). (H) Scatter plot showing basal body number located within 1 μm of the apical surface of MCCs induced by Multicilin, in wild-type control (Cont), Cas9/Foxj1gRNA1-injected (Foxj1) or Cas9/Foxn4gRNA1-injected (Foxn4) embryos based on scoring 12 fields from different embryos. Bars indicate the mean and s.d. Image published in: Campbell EP et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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