Xenbase Image ID: 156491

Fig. 4. RNAseq analysis of foxn4 and foxj1 mutant phenotypes. (A,B) The foxn4 and foxj1 sequences targeted by the Foxn4 and Foxj1 gRNAs, on chromosomes Chr1l and Ch9_10L, respectively. The sequence and location of the gRNAs are indicated, along with the coding frame, and location of the forkhead domain (shaded in red). Beneath are 20 randomly chosen sequence reads that map to these regions in an RNAseq analysis of progenitors from embryos injected with Cas9 protein and Foxn4gRNA1 (A) or Foxj1gRNA1 (B). (C) The total wild-type and mutant sequence reads observed at the two homeologs of the foxn4 and foxj1 genes in replicate RNAseq analysis of embryos injected with Cas9 protein and Foxn4gRNA1, Foxj1gRNA1, or the Foxn4 morpholino as a control. (D) Scatter plot of genes based on a log2-fold change in expression (P<0.05) in RNAseq analysis of progenitors induced to undergo MCC differentiation with Multicilin, in the presence of a Foxn4 morpholino, or Cas9/Foxn4gRNA1. Points in red are genes where Foxn4 binds directly within 1 kb of the TSS, based on ChIPseq analysis. (E-G) Scatter plots of genes based on log2-fold change in expression (P<0.05) in RNAseq analysis of progenitors induced to under MCC differentiation by Multicilin, in the presence and absence of E2f4δCT to disable the EDM complex (F,G), with Cas9/Foxj1gRNA1 to mutate foxj1 (E,F) or with Cas9/Foxn4gRNA1 to mutate foxn4 (E,G). All genes changes with P<0.05 are indicated in gray, MCC core genes defined in Quigley and Kintner (2016 preprint) are in blue, and genes associated with centriole biogenesis are in red (Ma et al., 2014). Image published in: Campbell EP et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

Return to previous page