XB-IMG-156493
Xenbase Image ID: 156493
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Fig. 6.
MCC differentiation in foxn4 and foxj1 double mutants. (A-D) Shown are representative confocal images of the skin in embryos that were injected with Cas9/Foxj1gRNA1 (B), Cas9/Foxn4gRNA1 (C) or both (D), or left uninjected as control (A). Cell membranes are labeled with mRFP (blue), basal bodies with Centrin4-GFP (green) and cilia with acetylated tubulin antibody (red). (A-D′) z-sections, with dashed lines indicating the apical location of docked basal bodies in control MCCs. Scale bars: 10 μm. (E) The frequency of all MCCs (scored based on centriole expansion, docked or undocked) and outer cells in the skin of embryos injected with Cas9 and gRNAs as indicated. No values differ significantly from control embryos. (F) MCCs in embryos injected with Cas9 and gRNAs as indicated were scored based on wild-type cilia extension (as in A), reduced cilia (as in B) or absent cilia (as in D). (E,F) Data are based on >19 randomly chosen fields (0.13×0.13 mm) from different embryos. n.d., not detected. Error bars indicate s.d.
Image published in: Campbell EP et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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