Xenbase Image ID: 156495

Figure S1: Regulation of foxn4 expression by Multicilin during MCC differentiation. (A) Summary of RNAseq data taken from Quigley and Kintner (2016, preprint) that was obtained from skin progenitors at stage 18 manipulated to change MCC differentiation, by expressing an activated form of Notch (ICD), an inhibitor of Notch (DBM), a dominant-negative mutant of Multicilin (dnMulticilin), or by ectopic activation of Multicilin (Multicilin). The role of the EDM complex in regulating foxn4 expression is also indicated by an RNAseq analysis that was carried out in isolated progenitors, expressing Multicilin alone or in the presence of a weak dominant-negative mutant of E2F4 described in Ma et al (Ma et al., 2014). Each condition was carried out as biological duplicates as one experiment indicated by the brackets below. The average normalized read numbers for the two forms of foxn4, rfx2 and foxj1 is shown. Error bars=S.D. Note the consistent read values for each conditions carried out independently. (B) Shown are screen shots of the Xenopus laevis genomic regions containing the long (L) and short (S) alleles for foxn4, along with ChIPseq and RNAseq results taken from Quigley and Kintner obtained from skin progenitors at stage 18. For the RNAseq experiments, MCC differentiation was repressed using activated Notch (ICD) or induced ectopically using Multicilin as described in experiment 3 in panel A. Image published in: Campbell EP et al. (2016) Copyright © 2016. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Larger Image Printer Friendly View

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