Xenbase Image ID: 1565

Fig. 3. Shh signalling down-regulates chd, resembling the effect of Notch. (A-E) In situ hybridisation of chd in early gastrulae, vegetal views, dorsal up, injected side on the right. (F-G′) In situ hybridisation of shh in neurulae, dorsal views, anterior up. (H) In situ hybridisation of N-tubulin and shh in neurulae. (I) In situ hybridisation of gli3 in neurulae. (J) External morphology at tadpole stages. (A-E) Embryo injected with (A) 2 ng of nuc-lacZ, (B) 1 ng of notchICD, (C) 2 ng of X-su(H)DBM, (D) 1 ng of X-shh mRNA, (E) 2 ng of X-shh-ds RNA. (F) Control embryo. (F′) Higher magnification of the embryo shown in F. (G) Embryo injected with 2 ng of X-shh-ds RNA to show the degradation of endogenous shh transcripts (77% of injected embryos, n=22). (G′) Higher magnification of the embryo shown in G. (H,I) Embryos injected with 2 ng of X-shh-ds RNA on the right side. (J) Control embryo (left) and two embryos injected with 1 ng of X-shh-ds RNA per blastomere at the 2-cell stage showing different grades of cyclopia (right). Image published in: López SL et al. (2003) Copyright © 2003. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License. Gene Synonyms Species Stage(s) Tissue chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10 blastopore upper blastopore lip shh.L hedgehog, vhh-1, X-SHH, Xhh, xshh X. laevis Throughout NF stage 15 neural groove pre-chordal neural plate chordal neural plate axial mesoderm tubb2b.S N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin X. laevis Throughout NF stage 15 neural plate anterior neural fold chordal neural plate pre-chordal neural plate neural tube gli3.L XGli-3, Xgli3 X. laevis Throughout NF stage 15 anterior neural fold pre-chordal neural plate Image source: Published Larger Image Printer Friendly View

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